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Recombinant Glutamate pyruvate transaminase from Bacillus subtilis

 Product Information

Cat #
MBS-1300
CAS No.
37277-85-3
Enzyme Commission Number
EC 2.6.1.21
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
D-aspartate transaminase; D-alanine aminotransferase; D-aspartic aminotransferase; D-alanine-D-glutamate transaminase; D-alanine transaminase; D-amino acid aminotransferase
Type
Function
A pyridoxal-phosphate protein. The enzyme from thermophilic Bacillus species acts on many D-amino acids with D-alanine and D-2-aminobutyrate as the best amino donors. It can similarly use any of several 2-oxo acids as amino acceptor, with 2-oxoglutarate and 2-oxobutyrate among the best. The enzyme from some other sources has a broader specificity.
Applications
Research Use
Storage
4°C
Storage Buffer
Shelf Life
Strains
Bacillus subtilis
Source
B. subtilis
Appearance
Molecular Weight
~ 33,346
Color / Form
Instruction
Enzyme Class
Transferases
Production Methods
Fermentation
Activity
66 U/mg protein at pH 7.5 and 25°C.
Specific Enzyme Activity
Purity
Unit Definition
One Unit of D-glutamate-pyruvate transaminase (D-GPT) is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Amino Acids Sequence
WARNINGS
Shipping
Formula
Reaction
D-alanine + 2-oxoglutarate = pyruvate + D-glutamate
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
On customer requests
Substrates
Concentration
2,000 U/mL
Usage And Dosage

 Description

Alanine transaminase (ALT) is a transaminase enzyme. It is also called alanine aminotransferase (ALAT) and was formerly called serum glutamate-pyruvate transaminase (SGPT) or serum glutamic-pyruvic transaminase (SGPT). ALT is found in plasma and in various body tissues, but is most common in the liver. It catalyzes the two parts of the alanine cycle. Serum ALT level, serum AST (aspartate transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.

For Research Use Only.
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