Enzyme Commission Number
EC 2.7.2.1
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
acetokinase; AckA; AK; acetic kinase; acetate kinase (phosphorylating)
Function
Requires Mg2+ for activity. While purified enzyme from Escherichia coli is specific for acetate, others have found that the enzyme can also use propanoate as a substrate, but more slowly. Acetate can be converted into the key metabolic intermediate acetyl-CoA by coupling acetate kinase with EC 2.3.1.8, phosphate acetyltransferase. Both this enzyme and EC 2.7.2.15, propionate kinase, play important roles in the production of propanoate.
Applications
Research Use
Strains
Bacillus stearothermophilus
Source
Bacillus stearothermophilus
Enzyme Class
Transferases
Production Methods
Fermentation
Activity
400-1,200 units/mg solid
Unit Definition
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30°C.
Reaction
ATP + acetate = ADP + acetyl phosphate
Specification
On customer requests
Description
In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. Short-chain fatty acids (SCFAs) play a major role in carbon cycle and can be utilized as a source of carbon and energy by bacteria. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydRate. A related enzyme, butyRate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyRate in the presence of ATP to form butyryl phosphate
