Products

Clostridium perfringens (C. welchii) Phospholipase C

Product Information

Cat #

MBS-0841

CAS No.

9001-86-9

Enzyme Commission Number

EC 3.1.4.3

Product Overview

High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.

Features

Ready-to-use product, accelerating research progress, enhancing application performance.

Method

Technology

Synonyms

lipophosphodiesterase I; lecithinase C; Clostridium welchii α-toxin; Clostridium oedematiens β- and γ-toxins; lipophosphodiesterase C; phosphatidase C; heat-labile hemolysin; α-toxin

Type

Function

The bacterial enzyme, which is a zinc protein, also acts on sphingomyelin and phosphatidylinositol; that from seminal plasma does not act on phosphatidylinositol.

Applications

Research Use

Storage

−20°C

Storage Buffer

Shelf Life

Strains

Clostridium spp.

Source

Clostridium perfringens (C. welchii)

Appearance

Molecular Weight

Color / Form

Instruction

Chromatographically purified

Enzyme Class

Hydrolases

Production Methods

Fermentation

Activity

Type I, > 150 units/mg protein; Type II, 10-50 units/mg protein; Type III, Type IV, > 1,000 units/mg protein (Lowry).

Specific Enzyme Activity

Purity

Unit Definition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37°C.

Amino Acids Sequence

WARNINGS

Shipping

Formula

Reaction

a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine

Recommendation

Species Reactivity

Contents

Compatibility

Melting Point

Final Titre

Fermentation Time

Recovery Yield

Starting Material

Specification

On customer requests

Substrates

Concentration

Usage And Dosage

Description

Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

For Research Use Only.

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