Enzyme Commission Number
EC 4.1.1.31
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
phosphopyruvate (phosphate) carboxylase; PEP carboxylase; phosphoenolpyruvic carboxylase; PEPC; PEPCase; phosphate:oxaloacetate carboxy-lyase (phosphorylating)
Function
This enzyme replenishes oxaloacetate in the tricarboxylic acid cycle when operating in the reverse direction. The reaction proceeds in two steps: formation of carboxyphosphate and the enolate form of pyruvate, followed by carboxylation of the enolate and release of phosphate.
Applications
Research Use
Production Methods
Fermentation
Activity
> 1 units/mg protein
Unit Definition
One unit will form 1.0 μmole of oxaloacetate from phospho (enol)pyruvate and CO2 per min at pH 8.5 at 25°C.
Reaction
phosphate + oxaloacetate = phosphoenolpyruvate + HCO3-
Specification
On customer requests