Products

Gluconobacter industrius D-Fructose Dehydrogenase

Product Information

Cat #

MBS-0454

CAS No.

37250-85-4

Enzyme Commission Number

EC 1.1.99.11

Product Overview

High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.

Features

Ready-to-use product, accelerating research progress, enhancing application performance.

Method

Technology

Synonyms

fructose 5-dehydrogenase (acceptor); D-fructose dehydrogenase; D-fructose:(acceptor) 5-oxidoreductase

Type

Function

2,6-Dichloroindophenol can act as acceptor.

Applications

Research Use

Storage

−20°C

Storage Buffer

Shelf Life

Strains

Gluconobacter industrius

Source

Gluconobacter industrius

Appearance

Molecular Weight

Color / Form

Instruction

Enzyme Class

Oxidoreductases

Production Methods

Fermentation

Activity

400-1,200 units/mg

Specific Enzyme Activity

Purity

Unit Definition

One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37°C.

Amino Acids Sequence

WARNINGS

Shipping

Formula

Reaction

D-fructose + acceptor = 5-dehydro-D-fructose + reduced acceptor

Recommendation

Species Reactivity

Contents

Compatibility

Melting Point

Final Titre

Fermentation Time

Recovery Yield

Starting Material

Specification

On customer requests

Substrates

Concentration

Usage And Dosage

Description

D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

For Research Use Only.

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