Enzyme Commission Number
EC 1.1.1.145
Product Overview
High-quality enzyme products.
Well-established quality management system.
Reaction
a 3β-hydroxy-Δ5-steroid + NAD+ = a 3-oxo-Δ5-steroid + NADH + H+
Systematic Name
3β-hydroxy-Δ5-steroid:NAD+ 3-oxidoreductase
Function
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Δ5-3-hydroxy steroids to a Δ4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3β-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Δ5 form to a Δ4 form (cf. EC 5.3.3.1, steroid Δ-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
Other name
progesterone reductase; Δ5-3β-hydroxysteroid dehydrogenase; 3β-hydroxy-5-ene steroid dehydrogenase; 3β-hydroxy steroid dehydrogenase/isomerase; 3β-hydroxy-Δ5-C27-steroid dehydrogenase/isomerase; 3β-hydroxy-Δ5-C27-steroid oxidoreductase; 3β-hydroxy-5-ene-steroid oxidoreductase; steroid-Δ5-3β-ol dehydrogenase; 3β-HSDH; 5-ene-3-β-hydroxysteroid dehydrogenase; 3β-hydroxy-5-ene-steroid dehydrogenase
Production Methods
Fermentation
Package
on customer request
Applications
Research Use
Storage
Should be stored in a dry and cool place, avoiding high temperature.
Appearance / Form
powder or liquid
Odor
Normal microbial fermentation odour.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
Description
Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. Our enzyme production services are based on bacteria, fungi, and yeast, from strain selection, optimization, and process development to scale-up production.
