Enzyme Commission Number
EC 3.6.1.71
Product Overview
High-quality enzyme products.
Well-established quality management system.
Reaction
(1) adenosine-5′-diphospho-5′-[DNA] + H2O = AMP + phospho-5′-[DNA]
(2) adenosine-5′-diphospho-5′-(ribonucleotide)-[DNA] + H2O = AMP + 5′-phospho-(ribonucleotide)-[DNA]
Systematic Name
adenosine-5′-diphospho-5′-[DNA] hydrolase (adenosine 5′-phosphate-forming)
Function
Aprataxin is a DNA-binding protein involved in different types of DNA break repair. The enzyme acts (among other activities) on abortive DNA ligation intermediates that contain an adenylate covalently linked to the 5′-phosphate DNA terminus. It also acts when the adenylate is covalently linked to the 5′-phosphate of a ribonucleotide linked to a DNA strand, which is the result of abortive ligase activty on products of EC 3.1.26.4, ribonuclease H, an enzyme that cleaves RNA-DNA hybrids on the 5′ side of the ribonucleotide found in the 5′-RNA-DNA-3′ junction. Aprataxin binds the adenylate group to a histidine residue within the active site, followed by its hydrolysis from the nucleic acid and eventual release, leaving a 5′-phosphate terminus that can be efficiently rejoined. The enzyme also possesses the activities of EC 3.6.1.70, guanosine-5′-diphospho-5′-[DNA] diphosphatase, and EC 3.6.1.72, DNA-3′-diphospho-5′-guanosine diphosphatase.
Other name
aprataxin; 5′-App5′-DNA adenylate hydrolase; APTX (gene name); HNT3 (gene name)
Production Methods
Fermentation
Package
on customer request
Applications
Research Use
Storage
Should be stored in a dry and cool place, avoiding high temperature.
Appearance / Form
powder or liquid
Odor
Normal microbial fermentation odour.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
Description
Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. Our enzyme production services are based on bacteria, fungi, and yeast, from strain selection, optimization, and process development to scale-up production.
