Enzyme Commission Number
EC 1.2.3.3
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
pyruvic oxidase; phosphate-dependent pyruvate oxidase
Function
A flavoprotein (FAD) requiring thiamine diphosphate. Two reducing equivalents are transferred from the resonant carbanion/enamine forms of 2-hydroxyethyl-thiamine-diphosphate to the adjacent flavin cofactor, yielding 2-acetyl-thiamine diphosphate (AcThDP) and reduced flavin. FADH2 is reoxidized by O2 to yield H2O2 and FAD and AcThDP is cleaved phosphorolytically to acetyl phosphate and thiamine diphosphate.
Applications
Diagnostic Industry
Storage
Store in tightly closed containers, desiccated, protected from light, at-20°C.
Strains
Aerococcus viridans
Source
Aerococcus viridans
Enzyme Class
Oxidoreductases
Production Methods
Fermentation
Reaction
pyruvate + phosphate + O2 = acetyl phosphate + CO2 + H2O2
Specification
On customer requests
Description
In enzymology, a pyruvate oxidase (EC 1.2.3.3) is an enzyme that catalyzes the chemical reaction: pyruvate + phosphate + O2 ↔ acetyl phosphate + CO2 + H2O2. The 3 substrates of this enzyme are pyruvate, phosphate, and O2, whereas its 3 products are acetyl phosphate, CO2, and H2O2.
