Akt kinase Activity Kit

 Product Information

Cat #
MBNK-02
CAS No.
Enzyme Commission Number
Product Overview
The Akt kinase activity kit provides all reagents for the detection of Akt kinase activity in cells. The kit is based on a solid-phase enzyme immunoassay for in vitro kinase detection by using a specific synthetic peptide as a substrate. The assay is designed for the analysis of Akt (1, 2, 3) activity in the solution phase.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Type
Function
Applications
Research Use
Storage
Store all components at 4°C, except active kinase at -80°C.
Storage Buffer
Shelf Life
Strains
Source
Appearance
Molecular Weight
Color / Form
Instruction
RUO - Research Use Only
Enzyme Class
Production Methods
Activity
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Shipped on Dry Ice
Formula
Reaction
Recommendation
Colorimetric detection, Activity assay
Species Reactivity
Species independent
Contents
Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard Specifications
Substrates
Concentration
Usage And Dosage

 Description

Akt plays a key role in the control of survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors and functions in a watermanin-sensitive pathway involving PI3 kinase. The once functionally elusive PDK2 phosphorylates Akt at Ser473, identified as the mammalian target of rapamycin (mTOR) in a non-sensitive complex containing Rictor and Sin1.target of rapamycin (mTOR)).

For Research Use Only.
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