Product Overview
The Caspase-3 colorimetric assay kit is used for the rapid detection of Caspase 3 in various samples. The assay principle is based on the fact that Caspase 3 hydrolyzes acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA), releasing the p-nitroanilide (pNA) fraction. The concentration of pNA released from the substrate can be calculated by measuring the absorbance value at 405 nm.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Applications
Research Use
Storage
Store Lysis Buffer, Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light.Long term storage at -20°C.
Instruction
RUO - Research Use Only
Shipping
Shipped on Blue Ice
Recommendation
Colorimetric detection,Activity assay
Contents
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl DEVD-pNA (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Specification
Standard Specifications