Enzyme Commission Number
EC 1.14.14.25
Product Overview
High-quality enzyme products.
Well-established quality management system.
Reaction
cholesterol + [reduced NADPH—hemoprotein reductase] + O2 = (24S)-cholest-5-ene-3β,24-diol + [oxidized NADPH—hemoprotein reductase] + H2O
Systematic Name
cholesterol,NADPH—hemoprotein reductase:oxygen oxidoreductase (24-hydroxylating)
Function
A P-450 heme-thiolate protein. The enzyme can also produce 25-hydroxycholesterol. In addition, it can further hydroxylate the product to 24,25-dihydroxycholesterol and 24,27-dihydroxycholesterol. This reaction is the first step in the enzymic degradation of cholesterol in the brain as hydroxycholesterol can pass the blood—brain barrier whereas cholesterol cannot. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH—hemoprotein reductase.
CAS No.
50812-30-1, 213327-78-7
Other name
cholesterol 24-monooxygenase; CYP46; CYP46A1; cholesterol 24S-hydroxylase; cytochrome P450 46A1
Production Methods
Fermentation
Package
on customer request
Applications
Research Use
Storage
Should be stored in a dry and cool place, avoiding high temperature.
Appearance / Form
powder or liquid
Odor
Normal microbial fermentation odour.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
Description
Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. Our enzyme production services are based on bacteria, fungi, and yeast, from strain selection, optimization, and process development to scale-up production.
