Enzyme Commission Number
EC 6.3.2.34
Product Overview
High-quality enzyme products.
Well-established quality management system.
Reaction
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme γ-F420-2
Systematic Name
L-glutamate:coenzyme F420-1 ligase (GDP-forming)
Function
This protein catalyses the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. In the first reaction (EC 6.3.2.31, coenzyme F420-0—L-glutamate ligase) the enzyme attaches a glutamate via its α-amine group to F420-0. In the second reaction, which is described here, the enzyme catalyses the addition of a second L-glutamate residue to the γ-carboxyl of the first glutamate.
Other name
F420:γ-glutamyl ligase; CofE-AF; MJ0768; CofE
Production Methods
Fermentation
Package
on customer request
Applications
Research Use
Storage
Should be stored in a dry and cool place, avoiding high temperature.
Appearance / Form
powder or liquid
Odor
Normal microbial fermentation odour.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
Description
Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. Our enzyme production services are based on bacteria, fungi, and yeast, from strain selection, optimization, and process development to scale-up production.