Product Overview
The HAT activity assay kit provides a rapid assay for HATase activity in a variety of samples using acetyl CoA and H4 histone peptides as substrates. In this assay, the HAT enzyme catalyzes the transfer of acetyl groups from acetyl CoA to histone peptides, resulting in two products - acetylated peptide and CoA-SH - which then serve as essential coenzymes for the production of NADH,which can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Applications
Research Use
Storage
Long term storage at -80°C.
Instruction
RUO - Research Use Only
Shipping
Shipped on Dry Ice
Recommendation
Colorimetric detection,Activity assay
Contents
2x HAT Assay Buffer,HAT Substrate I,HAT Substrate II,NADH Generating Enzyme,NE (Nuclear Extract 4 mg/ml),HAT Reconstitution Buffer
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Specification
Standard Specifications