Enzyme Commission Number
EC 2.8.2.30
Product Overview
High-quality enzyme products.
Well-established quality management system.
Reaction
3′-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3′,5′-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
Systematic Name
3′-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfonotransferase
Function
Two major substrates contain the tetrasaccharides: → undetermined 2-sulfo-uronic acid→ GlcN2S→ IdoA2S→ GlcN*→ and → undetermined 2-sulfo-uronic acid→ GlcN2S→ IdoA2S→ GlcN6S*→ (symbols as in 2-Carb-38) with modification of the N-unsubstituted glucosamine residue (shown with an asterisk). Modification of selected sequences containing N-sulfo-glucosamine residues cannot yet be excluded. The 3-O-sulfated heparan sulfate can be utilized by Herpes simplex virus type 1 as an entry receptor to infect the target cells. There are two isozymes, known as 3-OST-3A and 3-OST-3B, which have identical catalytic domains but are encoded by different mammalian genes. The specificity of this enzyme differs from that of the other [heparan sulfate]-glucosamine 3-sulfotransferases. It is inefficient at modifying precursors of the antithrombin binding site [in contrast to EC 2.8.2.23 ([heparan sulfate]-glucosamine 3-sulfotransferase 1)] and it does not modify glucosamine preceded by GlcA2S [unlike EC 2.8.2.29 ([heparan sulfate]-glucosamine 3-sulfotransferase 2)].
Other name
3′-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase
Production Methods
Fermentation
Package
on customer request
Applications
Research Use
Storage
Should be stored in a dry and cool place, avoiding high temperature.
Appearance / Form
powder or liquid
Odor
Normal microbial fermentation odour.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
Description
Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. Our enzyme production services are based on bacteria, fungi, and yeast, from strain selection, optimization, and process development to scale-up production.
