Products

Lys N Protease

Product Information

Cat #

MBS-1974

CAS No.

Enzyme Commission Number

Product Overview

Lys-N is an innovative protease that specifically hydrolyzes peptide bonds at the Nterminal of lysine residues. This enzyme can be applied for post-translational modification (PTM) protein research, and it produces more b ions than y ions in the spectrometer. Combing the use of Lys-N with rTrypsin-N gives the best way for protein sequencing.

Features

Ready-to-use product, accelerating research progress, enhancing application performance.

Method

Technology

Synonyms

Lys N; protease-N

Type

Function

Applications

Research Use

Storage

Lyophilized powder: stored in -20°C refrigerator; enzyme solution: stored at -20°C or -80°C

Storage Buffer

Shelf Life

12-24 months

Strains

Source

Appearance

lyophilized powder

Molecular Weight

18.4kDa

Color / Form

Instruction

Avoid inhalation during use, prevent contact with skin or mucous membranes, and rinse with water immediately after accidental contact.

Enzyme Class

Hydrolases

Production Methods

Activity

394 units/mg

Specific Enzyme Activity

Purity

High Purity (≥ 99.5%)

Unit Definition

One unit of proteolytic activity towards azocasein is defined as the amount of enzyme required for half-maximal OD366 after a 30 min incubation at 37 °C, pH 10, A366, and light path=1cm.

Amino Acids Sequence

WARNINGS

Shipping

Timely shipping by optional means

Formula

Reaction

Recommendation

Protease/Protein (w/w):1:30
Digest in a water bath at 37°C for 4h

Species Reactivity

Contents

Compatibility

Melting Point

Final Titre

Fermentation Time

Recovery Yield

Starting Material

Specification

Standard and Custom Specifications

Substrates

Concentration

Usage And Dosage

Description

Lys N Protease is a mirror protease complementary to Lys-N.

For Research Use Only.

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