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Biologics

Lys N Protease

 Product Information

Cat #
MBS-1974
CAS No.
Enzyme Commission Number
Product Overview
Lys-N is an innovative protease that specifically hydrolyzes peptide bonds at the Nterminal of lysine residues. This enzyme can be applied for post-translational modification (PTM) protein research, and it produces more b ions than y ions in the spectrometer. Combing the use of Lys-N with rTrypsin-N gives the best way for protein sequencing.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Lys N; protease-N
Type
Function
Applications
Research and Development
Storage
Lyophilized powder: stored in -20°C refrigerator; enzyme solution: stored at -20°C or -80°C
Storage Buffer
Shelf Life
12-24 months
Strains
Source
Appearance
lyophilized powder
Molecular Weight
18.4kDa
Color / Form
Instruction
Avoid inhalation during use, prevent contact with skin or mucous membranes, and rinse with water immediately after accidental contact.
Enzyme Class
Hydrolases
Production Methods
Activity
394 units/mg
Specific Enzyme Activity
Purity
High Purity (≥ 99.5%)
Unit Definition
One unit of proteolytic activity towards azocasein is defined as the amount of enzyme required for half-maximal OD366 after a 30 min incubation at 37 °C, pH 10, A366, and light path=1cm.
Amino Acids Sequence
WARNINGS
Shipping
Timely shipping by optional means
Formula
Reaction
Recommendation
Protease/Protein (w/w):1:30
Digest in a water bath at 37°C for 4h
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard and Custom Specifications
Substrates
Concentration
Usage And Dosage
Product Type
Enzymes

 Description

Lys N Protease is a mirror protease complementary to Lys-N.

For Research Use Only.
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