Enzyme Commission Number
EC 3.2.1.89
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
endo-1,4-β-galactanase; galactanase (ambiguous); arabinogalactanase; ganB (gene name)
Function
This enzyme, isolated from the bacterium Bacillus subtilis, hydrolyses the β(1→4) bonds found in type I plant arabinogalactans, which are a component of the primary cell walls of dicots. The predominant product is a tetrasaccharide. cf. EC 3.2.1.181, galactan endo-β-1,3-galactanase.
Applications
Research Use
Storage
The enzyme is supplied as an ammonium sulphate suspension is the presence of 0.02% sodium azide. On dilution in buffer, the enzyme should be stored in the frozen state between use.
Strains
Aspergillus niger
Production Methods
Fermentation
Activity
~ 400 U/mg at 40°C and pH 4.0.
Unit Definition
One Unit of activity is defined as the amount of enzyme required to release one micromole of reducing-sugar equivalents from the substrate employed at pH 4 and 40°C.
Reaction
The enzyme specifically hydrolyses (1→4)-β-D-galactosidic linkages in type I arabinogalactans.
Specification
On customer requests
