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Recombinant Heparinase II from Bacteroides eggerthii

 Product Information

Cat #
MBS-1469
CAS No.
9025-39-2
Enzyme Commission Number
EC 4.2.2.7
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
heparin eliminase; heparinase
Type
Function
Applications
Research and Development
Storage
at -80°C
Storage Buffer
Shelf Life
Strains
Escherichia coli
Source
E. coli
Appearance
Molecular Weight
86 kDa
Color / Form
Instruction
Enzyme Class
Lyases
Production Methods
Fermentation
Activity
Specific Enzyme Activity
Purity
> 95% determined by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme that will liberate 1.0 μmol unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0 in a total reaction volume of 100 μl.
Amino Acids Sequence
WARNINGS
Shipping
Formula
Reaction
Eliminative cleavage of polysaccharides containing (1→4)-linked D-glucuronate or L-iduronate residues and (1→4)-α-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-α-D-gluc-4-enuronosyl groups at their non-reducing ends
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
On customer requests
Substrates
Concentration
4,000 units/ml
Usage And Dosage
Product Type
Enzymes

 Description

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.

For Research Use Only.
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