Enzyme Commission Number
EC 4.2.2.2
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
polygalacturonic transeliminase; pectic acid transeliminase; polygalacturonate lyase; endopectin methyltranseliminase; pectate transeliminase; endogalacturonate transeliminase; pectic acid lyase; pectic lyase; α-1,4-D-endopolygalacturonic acid lyase; PGA lyase; PPase-N; endo-α-1,4-polygalacturonic acid lyase; polygalacturonic acid lyase; pectin trans-eliminase; Polygalacturonic acid trans-eliminase
Function
Favours pectate, the anion, over pectin, the methyl ester (which is the preferred substrate of EC 4.2.2.10, pectin lyase).
Applications
Research Use
Strains
Cellvibrio japonicus
Source
Cellvibrio japonicus
Molecular Weight
~ 38,035 Da
Production Methods
Fermentation
Activity
690 U/mg protein (on polygalacturonic acid) at pH 10.0 and 40°C.
Unit Definition
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of 4,5-unsaturated product per minute from polygalacturonic acid (1.25 mg/ml) in the presence of calcium chloride (1 mM) in CAPS buffer (50 mM) pH 10.0 and 40°C, monitored at 235 nm.
Reaction
Eliminative cleavage of (1→4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends
Specification
On customer requests
