Enzyme Commission Number
EC 2.3.1.193
Product Overview
High-quality enzyme products.
Well-established quality management system.
Reaction
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA + H2O = CoA + [elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate
Systematic Name
acetyl-CoA:[elongator tRNAMet]-cytidine34 N4-acetyltransferase (ATP-hydrolysing)
Function
The enzyme acetylates the wobble base cytidine34 of the CAU anticodon of elongation-specific tRNAMet. Escherichia coli TmcA strictly discriminates elongator tRNAMet from tRNAIle, which is structurally similar and has the same anticodon loop, mainly by recognizing the C27-G43 pair in the anticodon stem. The enzyme can use GTP in place of ATP for formation of N4-acetylcytidine.
Production Methods
Fermentation
Package
on customer request
Applications
Research Use
Storage
Should be stored in a dry and cool place, avoiding high temperature.
Appearance / Form
powder or liquid
Odor
Normal microbial fermentation odour.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
Description
Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. Our enzyme production services are based on bacteria, fungi, and yeast, from strain selection, optimization, and process development to scale-up production.
