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α-Amino Acid Ester Acyl Transferase

 Product Information

Cat #
MBS-2079
CAS No.
74506-40-4
Enzyme Commission Number
EC 3.1.1.43
Product Overview
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds.This product with the indicated enzyme activity was briefly purified from engineered E. coli. Genetic modifications (e.g. CRISPR/Cas9 System) through gene deletions, allele replacements, and the insertion of new genetic materials have been successfully used to create strains with better fermentation dynamics and stress tolerance.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
AST; α-amino acid ester hydrolase
Type
Crude Enzyme
Function
Applications
Research Use; Pharmaceutical Industry
Storage
Store at −20°C or colder.
Storage Buffer
Shelf Life
12 months
Strains
Escherichia coli
Source
Appearance
Solution
Molecular Weight
Color / Form
Instruction
Avoid inhalation during use, prevent contact with skin or mucous membranes, and rinse with water immediately after accidental contact.
Enzyme Class
Hydrolases
Production Methods
Recombinant Production
Activity
Undetermined
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Timely shipping by optional means
Formula
Reaction
an α-amino acid ester + H2O = an α-amino acid + an alcohol
Recommendation
Molecular biology and biochemistry research tool
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard and Custom Specifications
Substrates
Concentration
Usage And Dosage

 Description

The gene encoding α-amino acid ester acyl transferase (AET), the enzyme that catalyzes the peptide-forming reaction from amino acid methyl esters and amino acids, was cloned from Empedobacter brevis and Sphingobacterium siyangensis AJ2458 and expressed in Escherichia coli.

For Research Use Only.
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