Tel:

  Email:

  

Creative Biogene
Get A Quote
  1. Home
  2. Products
  3. Biomedicine
  4. Kit Development
  5. Bacillus polymyxa Neutral Protease (Dispase)
Products

Bacillus polymyxa Neutral Protease (Dispase)

 Product Information

Cat #
MBS-0741
CAS No.
9001-92-7
Enzyme Commission Number
EC 3.4.24.28
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Bacillus metalloendopeptidase; Bacillus subtilis neutral proteinase; anilozyme P 10; Bacillus metalloproteinase; Bacillus neutral proteinase; megateriopeptidase
Type
Function
Variants of this enzyme have been found in species of Bacillus including B. subtilis, B. amyloliquefaciens, B. megaterium (megateriopeptidase,), B. mesentericus, B. cereus and B. stearothermophilus. In peptidase family M4 (thermolysin family). Formerly included in EC 3.4.24.4
Applications
Research Use
Storage
Store at 2-8°C
Storage Buffer
Shelf Life
Strains
Bacillus polymyxa
Source
Bacillus polymyxa
Appearance
Molecular Weight
32.5 kDa
Color / Form
Instruction
Enzyme Class
Hydrolases
Production Methods
Fermentation
Activity
> 4 units per mg dry weight
Specific Enzyme Activity
Purity
Chromatographically purified
Unit Definition
One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate.
Amino Acids Sequence
WARNINGS
Shipping
Formula
Reaction
Similar, but not identical, to that of thermolysin
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
On customer requests
Substrates
Concentration
Usage And Dosage

 Description

Neutral protease (Dispase) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.

For Research Use Only.
Have a question? Get a Free Consultation