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Recombinant Endoglycosidase H

 Product Information

Cat #
MBS-1985
CAS No.
37278-88-9
Enzyme Commission Number
EC 3.2.1.96
Product Overview
Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Edo H; N,N′-diacetylchitobiosyl β-N-acetylglucosaminidase; endo-β-N-acetylglucosaminidase; mannosyl-glycoprotein endo-β-N-acetylglucosamidase; di-N-acetylchitobiosyl β-N-acetylglucosaminidase; endo-β-acetylglucosaminidase; endo-β-(1→4)-N-acetylglucosaminidase; mannosyl-glycoprotein 1,4-N-acetamidodeoxy-β-D-glycohydrolase; endoglycosidase S; endo-N-acetyl-β-D-glucosaminidase; endo-N-acetyl-β-glucosaminidase; endo-β-N-acetylglucosaminidase D; endo-β-N-acetylglucosaminidase F; endo-β-N-acetylglucosaminidase H; endo-β-N-acetylglucosaminidase L; glycopeptide-D-mannosyl-4-N-(N-acetyl-D-glucosaminyl)2-asparagine 1,4-N-acetyl-β-glucosaminohydrolase; mannosyl-glycoprotein endo-β-N-acetylglucosaminidase
Type
Function
Complete removal of N-linked high mannose from antibodies or other glycoproteins
Applications
Research Use
Storage
Store at -20 °C, 20mM Tris-HCl, 50mM NaCI, 5mM EDTA, pH 7.5
Storage Buffer
Shelf Life
12-24 months
Strains
Escherichia coli
Source
Streptomyces plicotus
Appearance
liquid
Molecular Weight
Color / Form
Instruction
Avoid inhalation during use, prevent contact with skin or mucous membranes, and rinse with water immediately after accidental contact.
Enzyme Class
Hydrolases
Production Methods
E. coli Fermentation
Activity
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Timely shipping by optional means
Formula
Reaction
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard and Custom Specifications
Substrates
Concentration
Usage And Dosage

 Description

Endoglycosidase H (Endo H) is an enzyme commonly used in biochemistry and molecular biology for the study of glycoproteins. It belongs to a class of enzymes known as glycosidases, which cleave glycosidic bonds in carbohydrates. Specifically, Endo H is used to cleave high-mannose and some hybrid-type N-linked oligosaccharides from glycoproteins.

For Research Use Only.
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