Enzyme Commission Number
EC 3.2.1.97
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
endo-α-acetylgalactosaminidase; endo-α-N-acetyl-D-galactosaminidase; mucinaminylserine mucinaminidase; D-galactosyl-3-(N-acetyl-α-D-galactosaminyl)-L-serine mucinaminohydrolase; endo-α-GalNAc-ase; glycopeptide α-N-acetylgalactosaminidase; D-galactosyl-N-acetyl-α-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase
Function
The enzyme catalyses the liberation of Gal-(1→3)-β-GalNAc α-linked to serine or threonine residues of mucin-type glycoproteins. EngBF from the bacterium Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1→3)-β-GalNAc. The enzymes from the bacteria Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. they can also release the core 2 trisaccharide Gal-(1→3)-β-(GlcNAc-(1→6)-β)-GalNAc or the core 3 disaccharide GlcNAc-(1→3)-β-GalNAc). The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan.
Applications
Research Use
Strains
Enterococcus faecalis
Source
Enterococcus faecalis
Molecular Weight
~ 158,800 Da
Production Methods
Fermentation
Activity
3.0 U/mg protein (on Gal-β-1,3-GalNAc-α-pNP) at pH 7.5 and 37°C
Unit Definition
One Unit of endo-α-N-acetylgalactosaminidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from Gal-β1,3-GalNAc-α-pNP (0.2 mM) in Tris.HCl buffer (10 mM) pH 7.5 and 37°C, monitored at 410 nm.
Reaction
β-D-galactosyl-(1→3)-N-acetyl-α-D-galactosaminyl-[glycoprotein]-L-serine/L-threonine + H2O = β-D-galactosyl-(1→3)-N-acetyl-D-galactosamine + [glycoprotein]-L-serine/L-threonine
Specification
On customer requests
