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Recombinant Protocatechuate 3,4-Dioxygenase from Bacteria

 Product Information

Cat #
MBS-1234
CAS No.
9029-47-4
Enzyme Commission Number
EC 1.13.11.3
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
protocatechuate oxygenase; protocatechuic acid oxidase; protocatechuic 3,4-dioxygenase; protocatechuic 3,4-oxygenase; protocatechuate:oxygen 3,4-oxidoreductase (decyclizing)
Type
Function
Requires Fe3+. The enzyme, which participates in the degradation of aromatic compounds, catalyses the intradiol addition of both oxygen atoms from molecular oxygen, resulting in ortho-cleavage of the aromatic ring. The type of cleavage leads to mineralization via the intermediate 3-oxoadipate.
Applications
Research Use
Storage
1 - 10°C (do not freeze)
Storage Buffer
Shelf Life
Strains
Escherichia coli
Source
E. coli
Appearance
Molecular Weight
28 kD α subuit, 24 kD β subunit（SDS-PAGE）
Color / Form
Instruction
Enzyme Class
Oxidoreductases
Production Methods
Fermentation
Activity
> 3 Units / mg
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Formula
Reaction
3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
On customer requests
Substrates
Concentration
Usage And Dosage

 Description

In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction:3,4-dihydroxybenzoate + O2↔ 3-carboxy-cis,cis-muconate. Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis,cis-muconate. This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, iron.

For Research Use Only.
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