Product Overview
The Carboxyfluorescein Multi-Caspase Activity Kit detects apoptosis through the covalent binding of a green fluorescent probe to active caspase enzymes in living cells. The carboxyfluorescein-labeled caspase inhibitor probe (FAM-VAD-FMK; excitation at 490 nm and emission at 520 nm), comprising a carboxyfluorescein derivative of valylalanylaspartic acid fluoromethyl ketone (VAD-FMK), covalently binds to the reactive cysteine (Cys 285) on the large subunit of active caspase heterodimers. This probe is both cell permeable and non-cytotoxic during the time of the labeling procedure and allows detection of apoptosis by fluorescence microscopy, flow cytometry, and 96-well-plate based fluorometry. Use of the fluorescence microscope provides a qualitative view of the level of apoptosis induced as compared to the use of a fluorometer or a flow cytometer which will generate quantitative data. The Sulforhodamine Multi-Caspase Activity Kit for Apoptosis Detection is a related kit that contains a sulforhodamine-labeled probe (Ex: 550, Em: 595).
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Applications
Research Use
Storage
Store the unopened kit (and each unopened component) at 2-8°C until the expiration date. Protect the FAM-VAD-FMK reagent from light at all times. Once reconstituted, the 150X FAM-VAD-FMK stock should be stored at -20°C protected from light. This reagent is stable for up to 6 months and may be thawed twice during that time.
Instruction
RUO - Research Use Only
Shipping
Shipped on Blue Ice
Recommendation
Colorimetric detection, HTS,Activity assay
Contents
FAM-VAD-FMK Reagent, 10X Wash Buffer, Fixative, Propidium Iodide.
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Specification
Standard Specifications
