Product Overview
The Caspase-1 colorimetric assay kit is used to determine the cystathione activity of the recognition sequence YVAD. The principle of the assay is based on the spectrophotometric detection of the chromophore p-nitroaniline after cleavage from the labeled substrate YVAD-pNA. A fold increase in cystein-1 activity can be determined by comparing the absorbance of pNA in treated samples with an untreated control.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Applications
Research Use
Storage
Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light.Long term storage at -20°C.
Instruction
RUO - Research Use Only
Shipping
Shipped on Blue Ice
Recommendation
Colorimetric detection,Activity assay
Contents
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl YVAD-pNA (1mM), 100µl DTT (1M), 25ml Dilution Buffer.
Compatibility
This product is compatible with the Absorbance 96 Plate Reader.
Specification
Standard Specifications