Horse Meat Speciation Nucleic Acid Detection Kit

 Product Information

Cat #
CBMN-DG212
CAS No.
Enzyme Commission Number
Product Overview
Biochemical assays for microbial pathogen detection work on the basis of polymerase chain reaction (PCR) or reverse-transcriptase PCR.
Features
Robust Lyophilized qPCR master mix;
Highly specific detection profile;
High priming efficiency;
Accurate controls.
Method
Technology
A real-time polymerase chain reaction (real-time PCR) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR). Two common methods for the detection of PCR products in real-time PCR are (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA and (7) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.
Synonyms
Type
Molecular detection for Meat/Fish Speciation
Function
Applications
Food Microbiology
Storage
• Store at -20°C
• Repeated thawing and freezing (>3x) should be avoided
• Cool all reagents during the working steps
Storage Buffer
Shelf Life
Strains
Source
Appearance
Molecular Weight
Color / Form
Instruction
Molecular detection for Meat/Fish Speciation
Enzyme Class
Production Methods
Activity
Specific Enzyme Activity
Purity
Unit Definition
Amino Acids Sequence
WARNINGS
Shipping
Shipping at room temperature
Formula
Reaction
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
Standard and Custom Specifications
Substrates
Concentration
Usage And Dosage

 Description

This kit provides a method for detecting Equus caballus mitochondrial DNA in food samples. The kit is supplied in a multiplex format enabling both species-specific and ‘universal animal’ detection in a single well. The relative signals produced by each of these targets will allow the user to calculate speciation percentage with minimal sample usage.

For Research Use Only.
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