Enzyme Commission Number
EC 1.1.3.13
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
ethanol oxidase; alcohol:oxygen oxidoreductase
Function
The enzymes from the fungi Candida methanosorbosa and several Basidiomycetes species contain an FAD cofactor. The enzyme from the phytopathogenic fungi Colletotrichum graminicola and Colletotrichum gloeosporioides utilize a mononuclear copper-radical mechanism. The enzyme acts on primary alcohols and unsaturated alcohols, and has much lower activity with branched-chain and secondary alcohols.
Applications
Research Use
Enzyme Class
Oxidoreductases
Production Methods
Fermentation
Activity
10-40 units/mg protein (biuret)
Unit Definition
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25°C.
Reaction
a primary alcohol + O2 = an aldehyde + H2O2
Specification
On customer requests
Description
In enzymology, an alcohol oxidase (EC 1.1.3.13) is an enzyme that catalyzes the chemical reaction:a primary alcohol + O2↔ an aldehyde + H2O2. Thus, the two substRates of this enzyme are primary alcohol and O2, whereas its two products are aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. It employs one cofactor, FAD.
