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Staph aureus V8 Protease (Endoproteinase Glu-C)

 Product Information

Cat #
MBS-0973
CAS No.
137010-42-5
Enzyme Commission Number
EC 3.4.21.19
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
V8 proteinase; endoproteinase Glu-C; staphylococcal serine proteinase
Type
Function
From Staphylococcus aureus strain V8. In appropriate buffer the specificity is restricted to Glu┼. In peptidase family S1 (trypsin family)
Applications
Research Use
Storage
Store at 2-8°C
Storage Buffer
Shelf Life
Strains
Staphylococcus aureus
Source
Staph aureus V8
Appearance
Molecular Weight
27 kDa (Drapeau 1978).
Color / Form
Instruction
Enzyme Class
Hydrolases
Production Methods
Fermentation
Activity
> 500 units per mg dry weight
Specific Enzyme Activity
Purity
Chromatographically purified
Unit Definition
One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate.
Amino Acids Sequence
WARNINGS
Shipping
Formula
Reaction
Preferential cleavage: Glu┼, Asp┼
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
On customer requests
Substrates
Concentration
Usage And Dosage

 Description

Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27 kDa daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO-and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau.

For Research Use Only.
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