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Tritirachium album Proteinase K

 Product Information

Cat #
MBS-0489
CAS No.
39450-01-6
Enzyme Commission Number
EC 3.4.21.64
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Method
Technology
Synonyms
Tritirachium alkaline proteinase; Tritirachium album serine proteinase; proteinase K; Tritirachium album proteinase K; endopeptidase K
Type
Function
From the mold Tritirachium album Limber. A peptidase of family S8 (subtilisin family) containing two disulfide bridges and one free Cys near the active site His. Formerly included in EC 3.4.21.14
Applications
Research Use
Storage
−20°C
Storage Buffer
Shelf Life
Strains
Tritirachium album
Source
Tritirachium album
Appearance
Molecular Weight
28.93 kDa
Color / Form
Instruction
Enzyme Class
Hydrolases
Production Methods
Fermentation
Activity
3.0-15.0 unit/mg solid; > 30 units/mg protein, ; > 800 units/mL; > 500 units/mL
Specific Enzyme Activity
Purity
Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37°C.
Amino Acids Sequence
WARNINGS
Shipping
Formula
Reaction
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyses peptide amides
Recommendation
Species Reactivity
Contents
Compatibility
Melting Point
Final Titre
Fermentation Time
Recovery Yield
Starting Material
Specification
On customer requests
Substrates
Concentration
Usage And Dosage

 Description

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments:pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

For Research Use Only.
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