Enzyme Commission Number
EC 1.11.1.13
Product Overview
High-quality enzyme products. Well-defined strains can be also provided for our clients to manufacture fermented products in a cost-effective way.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Synonyms
peroxidase-M2; Mn-dependent (NADH-oxidizing) peroxidase
Function
A hemoprotein. The enzyme from white rot basidiomycetes is involved in the oxidative degradation of lignin. The enzyme oxidizes a bound Mn2+ ion to Mn3+ in the presence of hydrogen peroxide. The product, Mn3+, is released from the active site in the presence of a chelator (mostly oxalate and malate) that stabilizes it against disproportionation to Mn2+ and insoluble Mn4+. The complexed Mn3+ ion can diffuse into the lignified cell wall, where it oxidizes phenolic components of lignin and other organic substrates. It is inactive with veratryl alcohol or nonphenolic substrates.
Applications
Research Use
Strains
Phanerochaete chrysosporium
Source
White-rot fungus (Phaner ochaete chrysosporium)
Instruction
This product is supplied as a mixture of isozymes, mol. mass (40-65 kDa).
Enzyme Class
Oxidoreductases
Production Methods
Fermentation
Unit Definition
One unit corresponds to the amount of enzyme, which oxidizes 1 μmole Mn2+ per minute to Mn3+ at pH 4.5 and 25°C
Reaction
2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O
Specification
On customer requests
